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Cytometry: new gates, scales, faster statistics, plot rendering and exports

2026.10.24-cytometrie-gates-echelles-exports · 2026-10-24

Delivered build : 534728a451d387e948434ad46e12417dd51acb13

Changes

  • Gates: the curvy quadrant (CurlyQuad) and the spider gate (Spider) join the existing shapes, with a final geometry computed by the server; ellipses and rectangles follow the usual cytometry conventions (rectangle open at the top of the axis). Gates of a sample group stay in sync.
  • Scales and parameters: Hyperlog scale, a biexponential scale, geometric mean, and derived parameters by formula (ratios, sums, logarithms), computed for local and hosted tubes; the entry screen only offers them on local tubes for now.
  • Statistics: median, percentiles, median absolute deviation and standard deviation, about six times faster on large tubes (2.7 million events: from 8.4 s to 1.3 s, identical results).
  • Plots: contour, zebra, density, palettes including Cividis, dot size, mouse-wheel or Shift + drag zoom, axis limits typed in the parameter's own unit, extra ticks when zoomed, histogram options, population overlay with true event counts and backgating.
  • Exports: PNG image at 1x, 2x or 4x, a lighter vector SVG, copy to clipboard, an A4 PDF sheet of every plot in the gallery, statistics as CSV.
  • Desktop app: heavy tubes are read in place on the computer, a disk cache makes reopening a tube almost instant, and the desktop app version shows in the sidebar.
  • Validated gates: a gate can be deleted until the study of its analysis is completed (the gate and its validations go, with an audit event); it locks once the study is completed, published, stopped or archived.

Known limitations

  • These tools stay off by default for existing teams (Annex D): an administrator turns them on team by team.
  • The biexponential scale is an empirical model: 94%, 85% and 81% of gates fall within 1% of the reference values on three control datasets, short of the 90% goal everywhere.
  • The curvy quadrant was not validated against a real reference file. Detailed statistics (standard deviation, median absolute deviation, percentiles, mode) have no exported reference.
  • No layout editor, tSNE, UMAP or batch processing yet. The new desktop app version is not published yet: the disk cache and the version display need that update.
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